Release note of BV_Analyzer Ver.0708
This document shows updated functions after Ver.0706
Summary of new functions of
Ver.0708
1. New analysis functions
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(1-1) |
The log file of
[Calculation] processes can be saved, loaded. |
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(1-2) |
The
exponential approximate curve is available in [Drift Remove] function. |
The exponential
approximate curve by 1-1/e(At) was added to [Drift remove]
function of [Calculation] tab page. |
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(1-3) |
[Stripe Show]
function was improved. Several
stripe maps are available, and the comparison of them is easier than
before. The information of the
points and the frame time are also available. |
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(1-4) |
AVI
replay speed can be set when AVI file is created and saved. |
When AVI file
of selected canvas area or image data is saved, the replay speed can be
input. User can set frame number
per second for AVI file. |
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(1-5) |
Bugs in
[Together by step num] and [Extraction by step num] functions are fixed. |
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2. Data acquisition functions
for MiCAM02
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(2-1) |
Acquisition can
be paused optionally during [Stim-No.Stim] mode. |
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(2-2) |
Acquired
data can be saved without result display on canvas. |
Acquired data
by sequencer can be saved without display on canvas in [AutoExe]
mode. |
1. New analysis functions
(1-1) Calculation log file is available;
[History File] area.
[History File] function was added
in [Calculation] tab page, so that the log of calculation processes can be
saved, and can be applied for other data.
* [Hist Save]: The
log of processes to selected data is displayed in [Calculation History] window
of [Calculation] tab page. [Hist Save] opens [Save as] window to save the log in .hst format. The
saved log file is binary format, so it can not be edited by general text
editors.
* [Hist Load]: This
button opens [Open file] window to select hst
file, and the content of selected hst file is
displayed before the loading. [OK]
button starts loading selected hst
file, and the file name is displayed under [Hist
Load] button. The software displays
last loading file name.
* [Apply All]: This
button starts processes in hst file to all data on
canvas.
(Attention) Only
the functions of [DF/F], [Spatial Filter], [Median Filter], [Cubic Filter],
[DERIV], [HPF/LPF] and [User Function(t)] can be
recorded in hst file.
(1-2) Exponential approximate curve in
[Drift Remove]
After the selection of data on
canvas, click [Drift Remove] of [Calculation] tab page.
*
Exponential approximate
curve was added to polynomial approximate curves selection. Check [1-1/exp(At)] in (2) of [Drift Remove] window to use exponential
approximate curve.
*
Set four reference
points, which colors are green, orange, gray or brown, on the areas with
remarkable drifting.
This function calculates the averaged wave, blue
wave, from the four points, and then the simple moving average wave is
calculated with 5 frames total, pre 2 frames and post 2 frames. 63% of the maximum value in the simple
moving average wave is calculated as time “T”. When A= 1/T, the drift curve, pink wave,
can be shown by F(y) = Max*(1-1/exp(Ax)). The coefficient “A” is common in this
data.
* Exponential curve can be controlled
manually.
On the wave in [Average & Drift Line (pink)],
horizontal pink lines shows maximum value and the 63% of it, and vertical pink
line shows the time constant point, which is 63% of the maximum value in
default. This time constant point
can be set manually by drag the vertical pink line.
(Attention) When
the reference points are changed or the preview point is changed, the
horizontal and vertical pink lines are set on default positions.
* [Preview] shows
the red approximate curve of the preview point, black point, as the preview
image of the result of removing drift process.
* Fitting check
box
ON: The vertical blue broken line, which is 10
frames wide, can be adjusted manually by drag to the point, which the value is
used as maximum value in above formula.
The averaged value of the 10 frames shown by the blue broken line is
used as the maximum value for the data.
Then the values from F(x, y, t) = Max(x, y)*81-1/exp(At))
is subtracted from the original data.
V’(x, y, t) = V(x, y, t) - F(x,
y, t)
V’(x, y, t)
is the result of Drift Remove, V(x, y, t) is preview point
OFF: 1/e level is calculated by the ratio of intensities
of the points of background image.
V'(x, y, t) = V(x, y, t) -
β*(1-1/exp(At))*backPrv/backRef
V’(x, y, t) is the result of
Drift Remove, V(x, y, t) is preview point, β*(1-1/exp(At))
is approximate curve from reference, backPrv is the
background intensity of Preview point, backRef is the
background intensity of Reference point.
* [Drift OK]
starts removing drift process to all pixel. After the end process, “Drift Remove
Exp” is shown in [Calculation History].
(1-3) Stripe Show
function was improved.
[Stripe Show] was improved, so
that image size is variable, and StripeMap can be
created in the set image size. Up
to 6 stripes can be set on same image, and the coordinates’ information of the
stripes is available.
* Select data on canvas, and
select [View]-[Display Window for Strip] in main menu, or click 
in tool bar to open [Show Stripe Main] Box.
(1) Select magnitude of image in [Image Zoom].
(2) Set the shape of stripe to [One Line],
[Bezier Curve] or [3 Bezier Curve].
(3) Up to 6 stripes can be set. Set [Stripe No.], and drag on the image
to make the stripe. [Bezier Curve]
needs the set of control points.
The start of the stripe is shown at (5), and the end is shown at
(6). In case of [One Line], the
length of the stripe is shown at (7) with pixel unit.
(4) After the setting of stripes on the image,
click [Map Draw] to open [Stripe Map] box.
The vertical axis of stripe map is special position on the original
image, and the horizontal axis is time, frame number.
[Roate]&[View] shows 3 dimensional display of stripe map, and
[Wave Display] shows waves on the stripe.
Those functions are same as before.
Up to 6 Stripe Maps are available in different window. To open different stripe map, change
[Stripe No.] and click [Map Draw].
New Function 1:The position on Strip Map links to
original data.
(A) Check [Image Point] of Stripe Map to show color lines on stripe map. The horizontal line can be moved by drag
on stripe map, and the position is shown in [Show Stripe Main] window.
New Function 2:
Frame position, time, line display
(B) Check [Frame(Time) Line] box to show the vertical
line on the stripe map. This line links
the time of image shown in [Show Stripe Main] window. The frame line on stripe map can be
moved by drag, and the image in [Show Stripe Main] window is automatically
changed according to the position of the frame line in [Stripe Map].
New Function 3: Detailed display in measure area
Input the size of pixel in [um/px]
to show the scale on the stripe map.
User can draw a line freely by drag on the stripe map, and the slope is
shown as the conductive velocity with the unit of micrometer per millisecond [um/ms]. The length of the free line and
difference of time are also displayed in (C).
New Function 4:
Movie function
[Movie] of [Control] tab page starts the movie in [Show Stripe Main]
window. The frame line, blue line,
in [Stripe Map] window is also moves according to the movie.
(1-4) AVI replay
speed can be set when AVI is created and saved.
* AVI replay speed
can be set when it exported. The
number of frame per second can be set, and the number should be more than 1.
* When the set
area of canvas is converted to AVI movie, the replay speed can be set on
[Export AVI] window.
* When AVI movie is created from
image data, the replay speed can be set in [Movie Export] window which is
opened by [Movie Export] of [Control] tab page.
* When 3D image
shown by [View]-[Display Window for 3D] is converted to AVI movie, the replay
speed can be set in [3D show] window.
(1-5) Others
* The bugs in
[Together by step num] and [Extraction by step num] functions are fixed.
2. Data acquisition function for
MiCAM02
(2-1) [Pause] function is
available in [Stim-Nostim] mode.
[Pause] of [Acquisition] window
starts pause status after next set of Stim-Nostim
acquisitions. This function is
available only in [Stim-Nostim] mode. [ReStart]
restarts the acquisition.
(2-2) Acquired data
can be saved without result display on canvas.
When [Save data without display
on canvas] of [Auto Exec by sequencer] window is checked, the acquired data is
saved without result display on canvas.
(Attention) “save_data” command must be in the
sequencer.
