Release Note of BV_Analyzer Ver0611
Major changes from Ver0606 are described here.
These are new functions.
1. The additions in analytical
functions. – These are
available for MiCAM Ultima/MiCAM02/MiCAM01 data -
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(1-1) |
New function which shows the area over
50% of the maximum amplitude in set-frame range. Also, whole of ActivationMap
image can be saved in BMP format. |
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(1-2) |
[Ref
Image Substitute] function adjusts pixel positions when MiCAM02 data are
used. |
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(1-3) |
[wave csv export] can output the
value up to three columns of decimal points. Also, [wave csv
export2(%)] function, which outputs difference as %
to the background value, is available. |
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(1-4) |
[Image csv
export] can export % of difference to the background-value, the
background-value and the difference-value. |
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(1-5) |
Continuous display of the difference value of the wave point |
Check
in [val] box in [Control] tab page shows the
information (values of the coordination, background and difference) of the
wave point(s) continuously. |
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(1-6) |
Preview
is available before the process of LowPassFilter or
HighPassFilter. Parameters can be checked by preview
before the processing. |
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(1-7) |
The
area, which user can set, on the canvas can be exported in AVI movie format. This function enables the export of
movie of set of image, wave and texts shown on the canvas. |
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(1-8) |
[DERIV]
button to calculate the first derivative was added in [Calculation] tab page. |
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(1-9) |
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2. An additional in acquisition
function – This is
available only for MiCAM02 -
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(2-1) |
New
function can display the monitor image in double size in [Acquisition]
window. |
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1. The additions in analytical functions. – These are available for MiCAM Ultima/MiCAM02/MiCAM01 data -
(1-1) Additional functions in [Activation Map]
* Until previous version, [ActivationMap] showed the time course of area of over-threshold. The new function in this version can detect maximum and minimum values of each pixel in set frame range, and can display the time course of the area which is over 50% of maximum amplitude.
* [BMP frame export] function is added. Although [BMP export] button in previous version could save BMP data of the set area and color bar (eg shown in the lower image in following figure), [BMP frame export] button can save whole image (eg shown upper image in following figure). When [interpolation] in [Control] tab page is un-checked, the data is saved in original size. And when it is checked, the image is saved at the magnification size shown at [ImageZoom] in [Control] tab page. When [Contour line] is not checked, the contour lines are not shown.
(1-2) Additional function in [Ref Image Substitute]
* [Ref Image Substitute] function can change background image, by which you can show the data of the difference at the higher sensitivity setting on the background image at higher spatial resolution setting. The image sizes of the difference and background are adjusted, and the difference data is overlaid on the background image. The position of the difference data on the background is adjusted as exact as possible.
(1-3) Additional function in [wave csv export]
* [wave csv export] in the pull down menu, which came up when the data was right clicked, exported integral number. But the numbers in three columns of decimal point are exported from this version.
Background Image : V(x, y), background data
Ref Image : D(x, y), difference data after the processes, such as [Pixel-filter], [Tcnst-Filter], [Cubic Filter] and so on.
* [Wave csv export2(%)] is added in the pull down menu, which came up when the data was right clicked. The displayed wave on the canvas is exported in % value. The percentage is the difference against the background value, and it is same as y scale on the canvas.
Background Image : (x, y) background data
Ref Image : D(x, y), difference data after the processes, such as [Pixel-filter], [Tcnst-Filter], [Cubic Filter] and so on.
% = -D(x, y)*100/(average number*V(x, y))
( !!! ) % here means the rate of difference value against the background value. When the data was not processed by [dF/F] in [Calculation] tab, % is against each pixel of the background. If the data was already processed by [dF/F], % is against the maximum value of the image, because the data was normalized already.
(1-4) Additional function in [image csv export]
* [Image csv export] in the pull down menu, which came up when the
data was right clicked, can export [Background Image], [Ref Image] and [Ref
Image (%)]. [Background Image]
shows the values of all pixels in the background image, [Ref Image] shows the difference
value from the background image, and [Ref Image (%)] shows % of difference to
the background value.
Background Image : (x, y) background value
Ref Image : D(x, y), difference value (raw value)
Ref Image (%) = -D(x, y)*100/(average number*V(x, y))
( !!! ) [Ref Image (%)] shows the rate of difference value against the background value. If the data was already processed by [dF/F], % is against the maximum value of the image, because the data was normalized already.
(1-5) Continuous display of the difference value of the wave point
* [val] function shows the pixel information, such as the coordinate, the background value and the difference value. When its box is checked, the pixel information is displayed continuously, and when its box is un-checked, the information is shown only when you are dragging the pixel on the image.
( !!! ) The difference value is the
division by the average number. And
it is the value of single pixel, so it may be not same as value shown in wave
when [Pixel-Filter] is more than “0”, or [Tcnst-Filter] is more than “1”, or [Cubic Filter] is on.
The pixel information display, [val] is on.
(1-6) Preview in LPF (Low
Pass Filter) and HPF (High Pass
* The preview function was added to LPF and HPF. You can check the parameter of the filter before the process.
The four waves from set points on the image are shown with each preview wave
(red line).
1) Set four points on the image.
2) Select filter (check button), LPF or HPF.
3) Adjust wave gain, and the parameter for filtering.
4) Check the results shown in red line.
5) Click “OK” button for the processing.
(!!!) The waves in this window is
that of raw data without any processing.
Their looks may differ from the waves on the canvas when [Pixel-Filter]
is more than “0”, or [Tcnst-Filter] is more than “1”, or [Cubic Filter] is on.
(1-7) Part of canvas can be exported in AVI
movie
* User can set area on the canvas, and the area can be exported in AVI format movie. The image and wave can be in a movie or several movies can be in a same movie using this function. Drag area which you want to make it movie. (The area shown by red line is for the movie.) Set the frame range and the steps, then click “OK”.
(1-8) The calculation of the first derivative
* [DERIV], which calculates the following first derivative, was added in [Calculation] tab page.
The algorithm of the first derivative calculation:
D(x, y, t): The coordinate (x, y), the difference at frame number “t”
D’(x, y, t): The result of the process
D’(x, y, t) = (D(x, y, t+1)-D(x, y, t-1))/2
But if t=0, D’(x, y, 0) = ((-3)*D(x, y, 0) + 4*D(x, y, 1) - D(x, y, 2))/2
When t = n-1 ( “n” shows the frame number)
D(x,y,n-1) = ( 3*D(x,y,n-1) - 4*D(x,y,n-2) + D(x,y,n-3) )/2
(1-9) Others
* The coordinate of [Reference Point] is shown in [Display Window for Statistic].
2. An additional in acquisition function – This is available only for MiCAM02 -
(2-1) large monitor image
The icon at lower right on the [Acquisition] window changes the size of monitor image to the normal size or large size. The large monitor image helps the adjustment of pixels in dual camera mode.
